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Influenza A H3N2 (A/Aichi/2/1968) Matrix protein 1/M1 Protein (His)

Catalog No. TMPY-00498

Influenza A H3N2 (A/Aichi/2/1968) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is I6SGW5.

Influenza A H3N2 (A/Aichi/2/1968) Matrix protein 1/M1 Protein (His)

Influenza A H3N2 (A/Aichi/2/1968) Matrix protein 1/M1 Protein (His)

Catalog No. TMPY-00498
Influenza A H3N2 (A/Aichi/2/1968) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is I6SGW5.
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Product Information

Biological Activity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Influenza A H3N2 (A/Aichi/2/1968) Matrix protein 1/M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is I6SGW5.
Species
H3N2
Expression System
E. coli
TagN-His
Accession NumberI6SGW5
Synonyms
cholinergic receptor, muscarinic 1
Construction
A DNA sequence encoding the influenza A virus (A/Aichi/2/1968(H3N2)) matrix protein 1 (AFM71858.1) (Met1-Lys252), termed as M1, was fused with a N-terminal polyhistidine tag. Predicted N terminal: His
Protein Purity
> 85 % as determined by SDS-PAGE
Molecular Weight30 kDa (predicted); 35 kDa (reducing conditions)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 1 mM EDTA, 50 mM Tris, 50 mM NaCl, 5% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
The Influenza virus matrix protein 1 (M1) is a matrix protein of the influenza virus. M1 protein has been shown to play a crucial role in virus replication, assembly, and budding. It forms a coat inside the viral envelope. This is a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M1 consists of two domains connected by a linker sequence. The N-terminal domain has a multi-helical structure. The C-terminal domain also contains an alpha-helical structure. The M1 protein is the most abundant structural protein in influenza A virus particles. M1 protein of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.

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