ED 50 < 50.0 ng/ml, measured by the cytotoxicity assay using M1 cells in presence of 10.0 ng/ml of human IL-6, corresponding to a specific activity of > 2.0 × 10 4 units/mg.

IL-6R alpha/CD126 Protein, Human, Recombinant (Insect, His) is expressed in Baculovirus Insect Cells. The accession number is P08887-1.

Human

Baculovirus Insect Cells

sIL6RA,Membrane glycoprotein 80,Interleukin-6 receptor subunit alpha,IL-6RA,IL-6R subunit alpha,IL-6R 1,gp80,CD_antigen: CD126

Leu20-Asp358

> 95% as determined by SDS-PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Interleukin-6 Receptor (IL-6R) is a single trans-membrane protein that is the receptor for Interleukin-6 (IL-6). IL-6R forms a hexameric complex upon binding 2 molecules of IL-6 and two molecules of glycoprotein 130 (gp130) which activates intracellular JAK/STAT pathways. Although the normal form of IL-6R is the membrane-bound 80 kDa subunit, a soluble form of IL-6R (sIL-6R) can be generated physiologically by limited proteolysis or alternative splicing. sIL-6R binds to both IL-6 and gp130 generating intracellular signaling. In the immune system, sIL-6R is produced by both naïve and memory CD4 T-cells and strongly augments IL-6 ligand’s induction of Th-17 cells.