HB-EGF Protein, Human, Recombinant (E. coli)

Name: HB-EGF Protein, Human, Recombinant (E. coli)
Brand: TargetMol
SKU: TMPS-00177
Rating: 4.5 (1 reviews)

(Synonyms: Heparin Binding EGF-like growth factor, HEGFL, HBEGF, DTR, Diphtheria toxin receptor) Copy Product Info

Synonyms: Heparin Binding EGF-like growth factor, HEGFL, HBEGF, DTR, Diphtheria toxin receptor

Catalog No. TMPS-00177 Copy Product Info

HB-EGF Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is Q99075.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$54	7-10 days	7-10 days
10 µg	$85	7-10 days	7-10 days
20 µg	$119	7-10 days	7-10 days
50 µg	$198	7-10 days	7-10 days
100 µg	$322	7-10 days	7-10 days
200 µg	$528	7-10 days	7-10 days
500 µg	$987	7-10 days	7-10 days
1 mg	$1,690	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	ED 50 < 0.75 ng/ml, measured in a cell proliferation assay using 3T3 cells.
Description	HB-EGF Protein, Human, Recombinant (E. coli) is expressed in E. coli. The accession number is Q99075.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	Q99075
Construction	Asp63-Leu148
Protein Purity	> 95% as determined by SDS-PAGE
Endotoxin	< 0.2 EU/μg of protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Heparin Binding EGF-like growth factor, HEGFL, HBEGF, DTR, Diphtheria toxin receptor
Research Background	Proheparin-binding EGF-like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, is a member of the EGF family of mitogens. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF signals through the EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared to EGF, HB-EGF binds to the EGF receptor with a higher affinity and has been shown to bemore mitogenic, likely due to its ability to bind to heparin and heparin sulfate proteoglycans. HB-EGF has also been reported to act as a diphtheria toxin receptor, mediating endocytosis of the bound toxin. Heparin-binding EGF-like growth factor has been shown to interact with NRD1, Zinc finger and BTB domain-containing protein 16 and BAG1.

Chemical Properties

Molecular Weight

9.7 kDa (Predicted); 12-14 kDa (Reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.