GCAP1 Protein, Human, Recombinant

Name: GCAP1 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-03554
Rating: 4.5 (1 reviews)

(Synonyms: GUCA1, GUCA, guanylate cyclase activator 1A (retina), GCAP1, GCAP, dJ139D8.6, CORD14, COD3, C6orf131) Copy Product Info

Synonyms: GUCA1, GUCA, guanylate cyclase activator 1A (retina), GCAP1, GCAP, dJ139D8.6, CORD14, COD3, C6orf131

Catalog No. TMPY-03554 Copy Product Info

GCAP1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 23.1 kDa and the accession number is P43080.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	GCAP1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 23.1 kDa and the accession number is P43080.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	P43080
Construction	A DNA sequence encoding the human GUCA1A (P43080)(Met1-Gly201) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 85 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	GUCA1, GUCA, guanylate cyclase activator 1A (retina), GCAP1, GCAP, dJ139D8.6, CORD14, COD3, C6orf131
Research Background	GCAP 1 gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messenger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase-activating protein 1 (GCAP 1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP 1 promotes the activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in GCAP 1 gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. GCAP 1 stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in the recovery of the dark state of rod photoreceptors following light exposure.

Chemical Properties

Molecular Weight

23.1 kDa (predicted); 20 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.