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G-CSF Protein, Human, Recombinant (Isoform 2) V2

Catalog No. TMPY-04829U Copy Product Info
G-CSF Protein, Human, Recombinant (Isoform 2) is expressed in E. coli. The accession number is P09919-2.

G-CSF Protein, Human, Recombinant (Isoform 2) V2

Catalog No. TMPY-04829U
Copy Product Info

G-CSF Protein, Human, Recombinant (Isoform 2) is expressed in E. coli. The accession number is P09919-2.

G-CSF Protein, Human, Recombinant (Isoform 2) V2
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$777-10 days7-10 days
10 μg$1257-10 days7-10 days
20 μg$2057-10 days7-10 days
50 μg$3397-10 days7-10 days
100 μg$4987-10 days7-10 days
200 μg$8767-10 days7-10 days
500 μg$1,8307-10 days7-10 days
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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)
For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Information

Biological Activity
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.03 ng/ml(Regularly tested).
Description
G-CSF Protein, Human, Recombinant (Isoform 2) is expressed in E. coli. The accession number is P09919-2.
Species
Human
Expression System
E. coli
TagTag Free
Accession NumberP09919-2
Synonyms
G-CSF,GCSF,CSF3OS,colony stimulating factor 3 (granulocyte),C17orf33OS,C17orf33
Construction
Thr31-Pro204
Protein Purity
> 95% as determined by SDS-PAGE
Molecular Weight18.8 kDa (Predicted); 16 kDa (Reducing conditions)
Endotoxin< 0.01 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
Reconstitution
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

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