G-CSF Protein, Human, Recombinant (Isoform 2) V2

Name: G-CSF Protein, Human, Recombinant (Isoform 2) V2
Brand: TargetMol
SKU: TMPY-04829U
Rating: 4.5 (1 reviews)

(Synonyms: G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33) Copy Product Info

Synonyms: G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33

Catalog No. TMPY-04829U Copy Product Info

G-CSF Protein, Human, Recombinant (Isoform 2) is expressed in E. coli. The accession number is P09919-2.

G-CSF Protein, Human, Recombinant (Isoform 2) V2

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Pack Size	Price	USA Stock	Global Stock
5 µg	$77	7-10 days	7-10 days
10 µg	$125	7-10 days	7-10 days
20 µg	$205	7-10 days	7-10 days
50 µg	$339	7-10 days	7-10 days
100 µg	$498	7-10 days	7-10 days
200 µg	$876	7-10 days	7-10 days
500 µg	$1,830	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.03 ng/ml(Regularly tested).
Description	G-CSF Protein, Human, Recombinant (Isoform 2) is expressed in E. coli. The accession number is P09919-2.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P09919-2
Construction	Thr31-Pro204
Protein Purity	> 95% as determined by SDS-PAGE
Endotoxin	< 0.01 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	G-CSF, GCSF, CSF3OS, colony stimulating factor 3 (granulocyte), C17orf33OS, C17orf33
Research Background	Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

Chemical Properties

Molecular Weight	18.8 kDa (Predicted); 16 kDa (Reducing conditions)
Relative Density.	no data available

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.