FGF-2 Protein, Human,Recombinant (Modified)

Name: FGF-2 Protein, Human,Recombinant (Modified)
Brand: TargetMol
SKU: TMPJ-00040
Price: 54 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Heparin-binding growth factor 2, HBGF-2, Fibroblast growth factor 2, FGFB, FGF-2, FGF2, bFGF, Basic fibroblast growth factor) Copy Product Info

Synonyms: Heparin-binding growth factor 2, HBGF-2, Fibroblast growth factor 2, FGFB, FGF-2, FGF2, bFGF, Basic fibroblast growth factor

Catalog No. TMPJ-00040 Copy Product Info

FGF-2 Protein, Human,Recombinant (Modified) is expressed in E. coli expression system. The predicted molecular weight is 17.2 kDa and the accession number is P09038-2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$54	-	In Stock
10 µg	$85	-	In Stock
20 µg	$118	-	In Stock
50 µg	$183	-	In Stock
100 µg	$287	7-10 days	7-10 days
200 µg	$449	7-10 days	7-10 days
500 µg	$825	7-10 days	7-10 days
1 mg	$1,180	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to induce FGF reporter activity in 293T human embryonic kidney cells.The ED50 for this effect is 0.46 ng/mL.
Description	FGF-2 Protein, Human,Recombinant (Modified) is expressed in E. coli expression system. The predicted molecular weight is 17.2 kDa and the accession number is P09038-2.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P09038-2
Amino Acid	Met1-Ser155
Construction	Met1-Ser155
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 EU/µg as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 5% sucrose, 0.02% Tween 80, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Heparin-binding growth factor 2, HBGF-2, Fibroblast growth factor 2, FGFB, FGF-2, FGF2, bFGF, Basic fibroblast growth factor
Research Background	Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule.

Chemical Properties

Molecular Weight

17.2 kDa (Predicted); 16 kDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.