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CD226 Protein, Cynomolgus, Recombinant (His)

TargetMol | SPR
Catalog No. TMPY-06192

CD226 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.7 kDa and the accession number is A0A2K5W1Z6-1.

CD226 Protein, Cynomolgus, Recombinant (His)

CD226 Protein, Cynomolgus, Recombinant (His)

TargetMol | SPR
Catalog No. TMPY-06192
CD226 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.7 kDa and the accession number is A0A2K5W1Z6-1.
Pack SizePriceAvailabilityQuantity
100 μg$6007-10 days
1 mg$3,9107-10 days
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Product Information

Biological Activity
Human CD155, hFc Tag  captured on CM5 Chip via Protein A can bind Cynomolgus DNAM-1, His Tag with an affinity constant of 0.82 μM as determined in SPR assay (Biacore T200).
Description
CD226 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.7 kDa and the accession number is A0A2K5W1Z6-1.
Species
Cynomolgus
Expression System
HEK293 Cells
TagC-His
Accession NumberA0A2K5W1Z6-1
Synonyms
CD226 molecule
Construction
Glu19-Phe252
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight27.7 kDa (Predicted); 48-68 kDa (Due to glycosylation)
EndotoxinLess than 1EU per μg by the LAL method.
FormulationLyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

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Please read the User Guide of Recombinant Proteins for more specific information.