Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His)

Catalog No. TMPY-00665 Copy Product Info

Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 104.2 kDa and the accession number is A0A024RC61.

Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His)

Catalog No. TMPY-00665

Copy Product Info

Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 104.2 kDa and the accession number is A0A024RC61.

Pack Size	Price	USA Stock	Global Stock
5 μg	$75	7-10 days	7-10 days
10 μg	$118	7-10 days	7-10 days
20 μg	$196	7-10 days	7-10 days
50 μg	$386	-	In Stock
100 μg	$660	7-10 days	7-10 days
200 μg	$1,120	7-10 days	7-10 days
500 μg	$2,270	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Information

Biological Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >2,500 pmoles/min/μg.
Description	Aminopeptidase N/CD13 Protein, Human, Recombinant (I603M, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 104.2 kDa and the accession number is A0A024RC61.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P15144
Synonyms	PEPN,P150,LAP1,GP150,CD13,APN,alanyl (membrane) aminopeptidase
Construction	A DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967，I603M) of the mature form of human ANPEP (NP_001141.2) was expressed with N-terminal signal peptide and C-terminal polyhistidine tag. Predicted N terminal: Lys 69
Protein Purity	> 97 % as determined by SDS-PAGE
Molecular Weight	104.2 kDa (predicted); 135-140 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.3. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.