CA125/MUC16 Protein, Human, Recombinant (hFc & AVI), Biotinylated

MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers. The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity. MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells. MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion. MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy. In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome.

Pack Size	Availability	Price/USD	Quantity
20 μg	5 days	$ 355.00
100 μg	5 days	$ 963.00

Pack Size

Availability

Price/USD

Quantity

20 μg

5 days

$ 355.00

100 μg

5 days

$ 963.00

Description

Species	Human
Expression System	Human Cells
Tag	C-Fc-Avi
Accession Number	Q8WXI7
Synonyms	mucin 16, cell surface associated, CA-125, CA125 ovarian cancer antigen, CA125, CA125MUC-16, mucin-16, MUC16, FLJ14303
Amino Acid	Gly12660-Met12923
Construction	Biotinylated Recombinant Human Mucin-16 is produced by our Mammalian expression system and the target gene encoding Gly12660-Met12923 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight	80-120 KDa, reducing conditions

Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 100 mM Glycine, pH 7.5.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Research Background	MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers. The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity. MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells. MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion. MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy. In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome.

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CA125/MUC16 Protein, Human, Recombinant (hFc & AVI), Biotinylated

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