Beta-NGF Protein, Mouse, Recombinant (HEK293)

Catalog No. TMPY-01402U

Beta-NGF Protein, Mouse, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.

Beta-NGF Protein, Mouse, Recombinant (HEK293)

Catalog No. TMPY-01402U

Beta-NGF Protein, Mouse, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.

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Biological Activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml. (QC Test) Immobilized Human Beta-NGF, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TrkA, His Tag with the EC50 of 0.25 μg/ml determined by ELISA.
Description	Beta-NGF Protein, Mouse, Recombinant (HEK293) is expressed in HEK293 Cells. The accession number is P01138.
Species	Human
Expression System	HEK293 Cells
Accession Number	P01138
Synonyms	Ngfb,nerve growth factor (β polypeptide),nerve growth factor (beta polypeptide)
Construction	Ser122-Ala241
Protein Purity	> 95% as determined by Bis-Tris PAGE
Molecular Weight	13.49 kDa (Predicted); 14-18 kDa (Due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in 20 mM NaAc, 150 mM Nacl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Nerve growth factor-beta (β-NGF) is a polypeptide growth factor that regulates survival, growth, and differentiation of specific peripheral and central neurons via its high-affinity receptor, tyrosine kinase receptor A (TrKA), and low-affinity receptor, p75 neurotrophin receptor (p75NTR).