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3CLpro/3C-like Protease Protein (A191V), SARS-COV-2, Recombinant

3CLpro/3C-like Protease Protein (A191V), SARS-COV-2, Recombinant
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3CLpro/3C-like Protease Protein (A191V), SARS-COV-2, Recombinant

Catalog No. TMPK-01342
3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CLpro/3C-like Protease Protein (A191V), SARS-COV-2, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 34.8kDa and the accession number is YP_009725301.1.
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Pack SizePriceAvailabilityQuantity
100 μg$8147-10 days
500 μg$3,2507-10 days
1 mg$5,4407-10 days
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Biological Description

Description
3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CLpro/3C-like Protease Protein (A191V), SARS-COV-2, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 34.8kDa and the accession number is YP_009725301.1.
Species
SARS-CoV-2
Expression System
E. coli
TagTag Free
Accession NumberYP_009725301.1
Synonyms
Mpro,3CL-Mpro,3CL Protease,M Proteinase
Construction
Ser1-Gln306(A191V)
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight34.8kDa (predicted) same as Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationSupplied as 0.22 μm filtered solution in 25 mM HEPES, 2.5 mM DTT, 10% Glycerol (pH 7.5).
Stability & Storage
It is recommended to store the product under sterile conditions at -70°C or lower. Samples are stable for up to 12 months at -80°C. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.