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Phosphatase Inhibitor Cocktail I (100× ddH2O)

Catalog No. C0002

It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.

The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Phosphatase Inhibitor Cocktail I (100× ddH2O)
Pack Size Availability Price/USD Quantity
1 mL In stock $ 29.00
1 mL * 10 In stock $ 205.00
1 mL * 50 In stock $ 841.00
1 mL * 100 In stock $ 1,236.00
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Composition

Ingredient CAS MW Concentration (100x) Target Type
Sodium Fluoride 7681-49-4 41.99 100 mM Acid Phosphatase Reversible
Sodium Orthovanadate 13721-39-6 183.91 100 mM Alkaline phosphatase, PTPs, ATPases Reversible
Sodium Molybdate 7631-95-0 205.92 115 mM Acid and phosphoprotein Phosphatase Irreversible
Sodium Tartrate 868-18-8 194.05 400mM Acid Phosphatase Reversible
Imidazole 288-32-4 68.08 200 mM Alkaline phosphatase Reversible

Features

  • Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
  • Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
  • Cost-Effective —— Lower price with higher quality

Handling Instruction

  • Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
  • Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
  • Briefly vortex cocktails to help facilitate the dissolution.

Notice

  • Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
  • If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.

Citations

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11. Lin H, Ai D, Liu Q, et al.Modified Podophyllotoxin Phenoxyacetamide Phenylacetate Derivatives: Tubulin/AKT1 Dual-Targeting and Potential Anticancer Agents for Human NSCLC.Journal of Natural Products.2023 12. Li W, Liang J, Li S, et al.The CXCL12-CXCR4-NLRP3 axis promotes Schwann cell pyroptosis and sciatic nerve demyelination in rats.Clinical and Experimental Immunology.2023: uxad081. 13. Yin H, Chen X, Chi Q, et al. The hybrid oncolytic peptide NTP-385 potently inhibits adherent cancer cells by targeting the nucleus. Acta Pharmacologica Sinica. 2022: 1-10. 14. Zhang B, Fan M, Fan J, et al. Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization. Microbiology Spectrum. 2022, 10(1): e02265-21. 15. Zhang B, Fan M, Fan J, et al. Avian hepatitis E virus ORF2 protein interacts with Rap1b to induce cytoskeleton 1 rearrangement that facilitates virus internalization 2. Microbiology Spectrum. 2022, 10(1): e02265-21 16. Tang H, Guo J, Sang B, et al. PDGFRβ Modulates Aerobic Glycolysis in Osteosarcoma HOS Cells via the PI3K/AKT/mTOR/c-Myc Pathway. Biochemistry and Cell Biology. 2021 (ja). 17. Zhu C, Zhang L, Zhao S, et al. UPF1 promotes chemoresistance to oxaliplatin through regulation of TOP2A activity and maintenance of stemness in colorectal cancer. Cell death & disease. 2021, 12(6): 1-15. 18. Wang L, Deng K, Gong L, et al. Chlorquinaldol targets the β-catenin and T-cell factor 4 complex and exerts anti-colorectal cancer activity. Pharmacological Research. 2020: 104955. 19. Zhang Y, Zhang J, Chen Y, et al. The ORF8 Protein of SARS-CoV-2 Mediates Immune Evasion through Potently Downregulating MHC-I. Proceedings of the National Academy of Sciences. 2021, 118(23). 20. Pei Y, Lv S, Shi Y, et al. RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1. Autophagy. 2022: 1-17 21. Liu F, Wang X, Duan J, et al. A Temporal PROTAC Cocktail‐Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells. Advanced Science. 2022: 2104823 22. Gao X, You J, Gong Y, et al. WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit. Acta Pharmaceutica Sinica B. 2021 23. Chen Q, Han H, Lin F, et al. Novel shikonin derivatives suppress cell proliferation, migration and induce apoptosis in human triple-negative breast cancer cells via regulating PDK1/PDHC axis. Life Sciences. 2022: 121077 24. Ren Y, Wang A, Wu D, et al. Dual inhibition of innate immunity and apoptosis by human cytomegalovirus protein UL37x1 enables efficient virus replication. Nature Microbiology. 2022: 1-13 25. Wang T, Wang D, Sun Y, et al.Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.Journal of Experimental & Clinical Cancer Research.2023, 42(1): 297. 26. Lin H, Ai D, Liu Q, et al.Natural isoflavone glabridin targets PI3Kγ as an adjuvant to increase the sensitivity of MDA-MB-231 to tamoxifen and DU145 to paclitaxel.The Journal of Steroid Biochemistry and Molecular Biology.2023: 106426. 27. Wang L, Deng R, Chen S, et al.Carboxypeptidase A4 negatively regulates HGS-ETR1/2-induced pyroptosis by forming a positive feedback loop with the AKT signalling pathway.Cell Death & Disease.2023, 14(12): 793. 28. Sun K, Sun J, Yan C, et al.Sympathetic Neurotransmitter, VIP, Delays Intervertebral Disc Degeneration via FGF18/FGFR2‐Mediated Activation of Akt Signaling Pathway.Advanced Biology.2023: 2300250. 29. Xu X, Yu Y, Zhang W, et al.SHP-1 inhibition targets leukaemia stem cells to restore immunosurveillance and enhance chemosensitivity by metabolic reprogramming.Nature Cell Biology.2024: 1-14.
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