Shopping Cart

Remove All
Your shopping cart is currently empty

Proceed to checkout Continue shopping

tdTomato mRNA

Name: tdTomato mRNA
Brand: TargetMol
SKU: TMRH-0005
Rating: 4.5 (1 reviews)

🥰Excellent

Catalog No. TMRH-0005

tdTomato-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency.

tdTomato mRNA

🥰Excellent

Catalog No. TMRH-0005

Product information is being updated, if you want to purchase, please click the bulk custom button.

Bulk & Custom

Questions

Resource Download

Product Introduction

Bioactivity

Description	tdTomato-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency.
In vitro	Fluorescence detection was performed 24 hours after transfecting HeLa cells with tdTomato mRNA (100 ng), and obvious red fluorescent signals were observed[1].
In vivo	tdTomato mRNA LNP-1 was injected intravenously into wild-type C57BL/6 mice at a dose of 0.3 mg/kg, and no tdTomato fluorescence was observed in the liver, spleen, or lungs after 24 hours, indicating that an alternative method for measuring mRNA translation in whole organs is necessary for LNP-1.[1].

Chemical Properties

Storage & Solubility Information

Storage

store at -80°C for 2 years

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc