Shepherdin is a synthetic peptide mirroring amino acids 79-87 of human survivin, acting as an inhibitor of the survivin-heat shock protein 90 (Hsp90) interaction. It specifically targets the N-terminal domain of Hsp90, blocking the survivin-Hsp90 interaction in human reticulocyte extracts at a 150 µM concentration. Conjugates of Shepherdin with cell-permeable peptides, such as penetratin or the HIV Tat sequence, exhibit anticancer properties both in vitro and in vivo.