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eGFP mRNA
🥰Excellent
Catalog No. TMRH-0003
eGFP-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency.
eGFP mRNA
eGFP mRNA
🥰Excellent
Catalog No. TMRH-0003
eGFP-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency.
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Product Introduction
Bioactivity
Chemical Properties
| Description | eGFP-mRNA is an mRNA molecule capped at the 5' end, polyadenylated at the 3' end, and modified with N1-Me-Pseudo UTP, mimicking the processed mature mRNA found in eukaryotes. The mRNA is capped co-transcriptionally using 3'-O-Me-GAG, forming a Cap1 structure which enhances mRNA stability and translation efficiency. The incorporation of the modified nucleotide N1-Me-Pseudo UTP reduces innate immune stimulation and increases mRNA stability. The addition of the poly(A) tail further stabilizes the mRNA and enhances its translation initiation efficiency. |
| In vitro | eGFP mRNA-LNP (1 μg/mL, 24 hours) can be efficiently translated into EGFP protein in HeLa cells, but exhibits very low translation efficiency in macrophages[1]. eGFP mRNA-LNP (0.25–1 μg/mL, 6–48 hours) showed dose- and time-dependent transfection efficiency in human primary airway epithelial cells and lung fibroblasts. At low doses (0.25 μg/mL), transfection peaked at 24 hours. At high doses (1 μg/mL), transfection efficiency was nearly the same between 24 and 48 hours[2]. |
| In vivo | In vivo imaging of C57Bl/6J mice 16 hours after a single intravenous injection of eGFP mRNA-LNP (2 mg/kg) revealed that eGFP mRNA was only translated into protein in hepatocytes, with no significant translation signals observed in Kupffer cells (KC). |
Storage & Solubility Information
| Storage | store at -80°C for 2 years |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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