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Anti-Wnt5a Polyclonal Antibody

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Catalog No. TMAB-01986
Alias WNT5A, Protein Wnt-5a

Anti-Wnt5a Polyclonal Antibody is a Rabbit antibody targeting Wnt5a. Anti-Wnt5a Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Wnt5a Polyclonal Antibody

Anti-Wnt5a Polyclonal Antibody

😃Good
Catalog No. TMAB-01986Alias WNT5A, Protein Wnt-5a
Anti-Wnt5a Polyclonal Antibody is a Rabbit antibody targeting Wnt5a. Anti-Wnt5a Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-Wnt5a Polyclonal Antibody is a Rabbit antibody targeting Wnt5a. Anti-Wnt5a Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasWNT5A, Protein Wnt-5a
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Rabbit)
Verified Activity
1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-WNT5A Polyclonal Antibody, Unconjugated (TMAB-01986) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Formalin-fixed and paraffin embedded human breast labeled with Rabbit Anti-WNT5A Polyclonal Antibody (TMAB-01986) at 1:250 at room temperature overnight followed by conjugation to secondary antibody.
3. Sample: Ovary (Mouse) Lysate at 30 μg
Primary: Anti-WNT5A (TMAB-01986) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 47 kDa
4. Sample: Uters (Mouse) Lysate at 30 μg
Primary: Anti-WNT5A (TMAB-01986) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 47 kDa
5. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WNT5A) Polyclonal Antibody, Unconjugated (TMAB-01986) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-WNT5A antibody (TMAB-01986)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
7. Sample:
Lane 1: Embryo (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Uterus (Mouse) Lysate at 40 μg
Lane 4: Uterus (Rat) Lysate at 40 μg
Lane 5: Ovary (Rat) Lysate at 40 μg
Lane 6: Vas deferens (Rat) Lysate at 40 μg
Primary: Anti-WNT5A (TMAB-01986) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human WNT5A
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
PTC & Wnt pathway,Neural Signal Transduction,Secreted
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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