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Anti-Wnt5a Polyclonal Antibody is a Rabbit antibody targeting Wnt5a. Anti-Wnt5a Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-Wnt5a Polyclonal Antibody is a Rabbit antibody targeting Wnt5a. Anti-Wnt5a Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Alias | WNT5A, Protein Wnt-5a |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Rabbit) |
Verified Activity | 1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-WNT5A Polyclonal Antibody, Unconjugated (TMAB-01986) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Formalin-fixed and paraffin embedded human breast labeled with Rabbit Anti-WNT5A Polyclonal Antibody (TMAB-01986) at 1:250 at room temperature overnight followed by conjugation to secondary antibody. 3. Sample: Ovary (Mouse) Lysate at 30 μg Primary: Anti-WNT5A (TMAB-01986) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 47 kDa 4. Sample: Uters (Mouse) Lysate at 30 μg Primary: Anti-WNT5A (TMAB-01986) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 47 kDa 5. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WNT5A) Polyclonal Antibody, Unconjugated (TMAB-01986) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-WNT5A antibody (TMAB-01986) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 7. Sample: Lane 1: Embryo (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Uterus (Mouse) Lysate at 40 μg Lane 4: Uterus (Rat) Lysate at 40 μg Lane 5: Ovary (Rat) Lysate at 40 μg Lane 6: Vas deferens (Rat) Lysate at 40 μg Primary: Anti-WNT5A (TMAB-01986) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]. |
Immunogen | KLH conjugated synthetic peptide: human WNT5A |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | PTC & Wnt pathway,Neural Signal Transduction,Secreted |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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