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Anti-VAMP8 Antibody (4K35)

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Catalog No. TMAC-04218
Alias Vesicle-associated membrane protein 8, Vesicle associated membrane protein 8, VAMP8_HUMAN, VAMP 8, Endobrevin, EDB

Anti-VAMP8 Antibody (4K35) is a Rabbit antibody targeting VAMP8. Anti-VAMP8 Antibody (4K35) can be used in WB,ICC/IF,IHC,IP,FCM.

Anti-VAMP8 Antibody (4K35)

Anti-VAMP8 Antibody (4K35)

😃Good
Catalog No. TMAC-04218Alias Vesicle-associated membrane protein 8, Vesicle associated membrane protein 8, VAMP8_HUMAN, VAMP 8, Endobrevin, EDB
Anti-VAMP8 Antibody (4K35) is a Rabbit antibody targeting VAMP8. Anti-VAMP8 Antibody (4K35) can be used in WB,ICC/IF,IHC,IP,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-VAMP8 Antibody (4K35) is a Rabbit antibody targeting VAMP8. Anti-VAMP8 Antibody (4K35) can be used in WB,ICC/IF,IHC,IP,FCM.
SynonymsVesicle-associated membrane protein 8, Vesicle associated membrane protein 8, VAMP8_HUMAN, VAMP 8, Endobrevin, EDB
Ig Type
IgG
Clone
4K35
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Mouse kidney.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
5. ICC staining VAMP8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining VAMP8 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining VAMP8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with VAMP8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-100; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundSyntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAPs (soluble NSF attachment proteins) and Synaptotagmin. Endobrevin, also designated VAMP-8 or ED, is a 100 amino acid single-pass type IV membrane protein that belongs to the synaptobrevin family. Similar in sequence to the synaptobrevins, endobrevin is abundantly expressed in kidney, moderately expressed in heart and spleen, and slightly expressed in brain, thymus and liver. Endobrevin interacts specifically with the SNAPs, most likely through an endobrevin-containing SNARE complex.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 11 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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