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Anti-VAMP2 Antibody (1D488)
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Catalog No. TMAC-04216
Alias Vesicle-associated membrane protein 2, VAMP-2, Vamp2, Synaptobrevin-2, Syb2
Anti-VAMP2 Antibody (1D488) is a Rabbit antibody targeting VAMP2. Anti-VAMP2 Antibody (1D488) can be used in WB,IP,ICC/IF,FCM.
Anti-VAMP2 Antibody (1D488)
Anti-VAMP2 Antibody (1D488)
😃Good
Catalog No. TMAC-04216Alias Vesicle-associated membrane protein 2, VAMP-2, Vamp2, Synaptobrevin-2, Syb2
Anti-VAMP2 Antibody (1D488) is a Rabbit antibody targeting VAMP2. Anti-VAMP2 Antibody (1D488) can be used in WB,IP,ICC/IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-VAMP2 Antibody (1D488) is a Rabbit antibody targeting VAMP2. Anti-VAMP2 Antibody (1D488) can be used in WB,IP,ICC/IF,FCM. |
| Synonyms | Vesicle-associated membrane protein 2, VAMP-2, Vamp2, Synaptobrevin-2, Syb2 |
| Ig Type | IgG |
| Clone | 1D488 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of VAMP2 on different cell lysates using anti-VAMP2 antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY5Y, Lane 2: Jurkat. 2. ICC staining VAMP2 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining VAMP2 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining VAMP2 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY5Y cells with VAMP2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and γ-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exo-cytosis by competing with SNAP 25 and syntaxins for VAMP binding. |
| Conjucates | Unconjugated |
| Immunogen | A synthesized peptide: within Human VAMP2 aa 9-42 / 116 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 15 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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