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Anti-ULK1 Antibody (6P282)
😃Good
Catalog No. TMAC-04195
Alias Unc-51-like kinase 1, Unc-51 like kinase 1(C. elegans), UNC51, Unc 51(C. elegans) like kinase 1, Unc 51 like kinase 1, UNC 51, ULK1, ULK 1, Serine/threonine protein kinase Unc51.1, Serine/threonine protein kinase ULK1, hATG1, Autophagy-related protein 1 homolog, ATG1A, ATG1, ATG 1
Anti-ULK1 Antibody (6P282) is a Rabbit antibody targeting ULK1. Anti-ULK1 Antibody (6P282) can be used in WB,IHC.
Anti-ULK1 Antibody (6P282)
Anti-ULK1 Antibody (6P282)
😃Good
Catalog No. TMAC-04195Alias Unc-51-like kinase 1, Unc-51 like kinase 1(C. elegans), UNC51, Unc 51(C. elegans) like kinase 1, Unc 51 like kinase 1, UNC 51, ULK1, ULK 1, Serine/threonine protein kinase Unc51.1, Serine/threonine protein kinase ULK1, hATG1, Autophagy-related protein 1 homolog, ATG1A, ATG1, ATG 1
Anti-ULK1 Antibody (6P282) is a Rabbit antibody targeting ULK1. Anti-ULK1 Antibody (6P282) can be used in WB,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Antigen Details
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| Description | Anti-ULK1 Antibody (6P282) is a Rabbit antibody targeting ULK1. Anti-ULK1 Antibody (6P282) can be used in WB,IHC. |
| Synonyms | Unc-51-like kinase 1, Unc-51 like kinase 1(C. elegans), UNC51, Unc 51(C. elegans) like kinase 1, Unc 51 like kinase 1, UNC 51, ULK1, ULK 1, Serine/threonine protein kinase Unc51.1, Serine/threonine protein kinase ULK1, hATG1, Autophagy-related protein 1 homolog, ATG1A, ATG1, ATG 1 |
| Ig Type | IgG |
| Clone | 6P282 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of ULK1 on different cell lysate using anti-ULK1 antibody at 1/1,000 dilution. Positive control: Lane1: MCF-7, Lane2: Mouse spleen, Lane3: SH-SY5Y. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ULK1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat skeleton muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ULK1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ULK1 antibody. Counter stained with hematoxylin. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 112 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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