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Anti-UBB Antibody (7M837)
😃Good
Catalog No. TMAC-02295
Alias UBB, Polyubiquitin-B
Anti-UBB Antibody (7M837) is a Rabbit antibody targeting UBB. Anti-UBB Antibody (7M837) can be used in WB,ICC/IF,IHC.
Anti-UBB Antibody (7M837)
Anti-UBB Antibody (7M837)
😃Good
Catalog No. TMAC-02295Alias UBB, Polyubiquitin-B
Anti-UBB Antibody (7M837) is a Rabbit antibody targeting UBB. Anti-UBB Antibody (7M837) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-UBB Antibody (7M837) is a Rabbit antibody targeting UBB. Anti-UBB Antibody (7M837) can be used in WB,ICC/IF,IHC. |
| Synonyms | UBB, Polyubiquitin-B |
| Ig Type | IgG |
| Clone | 7M837 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin. 5. ICC staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining K63-linkage Specific Ubiquitin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining K63-linkage Specific Ubiquitin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S Proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lys 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκB-α and p27. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 60-100 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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