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Anti-TSG101 Polyclonal Antibody

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Catalog No. TMAB-01910

Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TSG101 Polyclonal Antibody

Anti-TSG101 Polyclonal Antibody

😃Good
Catalog No. TMAB-01910
Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
Lane1: Brain (Rat) Lysate at 30 μg
Lane2: Heart (Rat) Lysate at 30 μg
Primary: Anti-Tsg101 (TMAB-01910) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G) at 1: 3000 dilution;
Predicted band size: 43 kDa
Observed band size: 43 kDa
5. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Primary:
Anti-TSG101 (TMAB-01910) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
6. Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 2: 293T (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Tsg101 (TMAB-01910) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Membrane; Peripheral membrane protein. Nucleus. Late endosome membrane; Peripheral membrane protein. Note=Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Tsg101
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cell cycle inhibitors,Cell differentiation,Cell Cycle Inhibitors,Cell Cycle Inhibitors,Cell Differentiation,Host Virus Interaction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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