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Anti-TSG101 Polyclonal Antibody
😃Good
Catalog No. TMAB-01910
Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-TSG101 Polyclonal Antibody
Anti-TSG101 Polyclonal Antibody
😃Good
Catalog No. TMAB-01910
Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TSG101 Polyclonal Antibody is a Rabbit antibody targeting TSG101. Anti-TSG101 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Tsg101 Polyclonal Antibody, Unconjugated (TMAB-01910) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Lane1: Brain (Rat) Lysate at 30 μg Lane2: Heart (Rat) Lysate at 30 μg Primary: Anti-Tsg101 (TMAB-01910) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G) at 1: 3000 dilution; Predicted band size: 43 kDa Observed band size: 43 kDa 5. Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 μg Primary: Anti-TSG101 (TMAB-01910) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa 6. Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 2: 293T (Human) Cell Lysate at 30 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Primary: Anti-Tsg101 (TMAB-01910) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Late endosome membrane; Peripheral membrane protein. Note=Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Tsg101 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cell cycle inhibitors,Cell differentiation,Cell Cycle Inhibitors,Cell Cycle Inhibitors,Cell Differentiation,Host Virus Interaction |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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