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Anti-TOMM20 Polyclonal Antibody
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Catalog No. TMAB-01878
Anti-TOMM20 Polyclonal Antibody is a Rabbit antibody targeting TOMM20. Anti-TOMM20 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-TOMM20 Polyclonal Antibody
Anti-TOMM20 Polyclonal Antibody
😃Good
Catalog No. TMAB-01878
Anti-TOMM20 Polyclonal Antibody is a Rabbit antibody targeting TOMM20. Anti-TOMM20 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TOMM20 Polyclonal Antibody is a Rabbit antibody targeting TOMM20. Anti-TOMM20 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | Sample: Lane 1: Mouse Kidney Lysates Lane 2: Mouse Brain Lysates Lane 3: Mouse Heart Lysates Lane 4: Mouse NIH/3T3 Lysates Lane 5: Rat Kidney Lysates Lane 6: Rat Brain Lysates Lane 7: Rat Heart Lysates Lane 8: Human Hela cell Lysates Lane 9: Human THP-1 cell Lysates Lane 10: Human HL-60 cell Lysates Lane 11: Human 293T cell Lysates Primary: Anti-TOMM20 (TMAB-01878) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion outer membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane (1). The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria (2). The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP (3,4). In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria (6,7). The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes (2,8) |
| Immunogen | KLH conjugated synthetic peptide: human TOM20 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Mitochondrial Biogenesis,Mitochondrial markers,Mitochondrial,Regulation |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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