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Anti-TLR3 Polyclonal Antibody

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Catalog No. TMAB-01854

Anti-TLR3 Polyclonal Antibody is a Rabbit antibody targeting TLR3. Anti-TLR3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-TLR3 Polyclonal Antibody

Anti-TLR3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01854
Anti-TLR3 Polyclonal Antibody is a Rabbit antibody targeting TLR3. Anti-TLR3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-TLR3 Polyclonal Antibody is a Rabbit antibody targeting TLR3. Anti-TLR3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig)
Verified Activity
1. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TLR3 Polyclonal Antibody, Unconjugated (TMAB-01854) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TLR3) Polyclonal Antibody, Unconjugated (TMAB-01854) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Placenta (Mouse) Lysate at 40 μg
Lane 2: Jurkat (Human) Cell Lysate at 30 μg
Lane 3: Hela (Human) Cell Lysate at 30 μg
Lane 4: Molt-4 (Human) Cell Lysate at 30 μg
Primary: Anti-TLR3 (TMAB-01854) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 120 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TLR3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Antiviral Signaling,Receptors,Beta Chemokines (CC),Macrophage / Inflamm.,TLR Signaling,Host Virus Interaction,SARS Coronavirus,NFkB Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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