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Anti-TAZ Polyclonal Antibody

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Catalog No. TMAB-01810
Alias WWTR1, WWTR 1, WW domain containing transcription regulator protein 1, WW domain containing transcription regulator 1, Transcriptional coactivator with PDZ binding motif, Transcriptional co activator with PDZ binding motif

Anti-TAZ Polyclonal Antibody is a Rabbit antibody targeting TAZ. Anti-TAZ Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-TAZ Polyclonal Antibody

Anti-TAZ Polyclonal Antibody

😃Good
Catalog No. TMAB-01810Alias WWTR1, WWTR 1, WW domain containing transcription regulator protein 1, WW domain containing transcription regulator 1, Transcriptional coactivator with PDZ binding motif, Transcriptional co activator with PDZ binding motif
Anti-TAZ Polyclonal Antibody is a Rabbit antibody targeting TAZ. Anti-TAZ Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-TAZ Polyclonal Antibody is a Rabbit antibody targeting TAZ. Anti-TAZ Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasWWTR1, WWTR 1, WW domain containing transcription regulator protein 1, WW domain containing transcription regulator 1, Transcriptional coactivator with PDZ binding motif, Transcriptional co activator with PDZ binding motif
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (TMAB-01810) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (TMAB-01810) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
MCF-7 (Human) Cell Lysate at 40 μg
LOVO (Human) CellLysate at 40 μg
A549 (Human) CellLysate at 40 μg
Primary: Anti-TAz (TMAB-01810) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 46 kDa
4. Blank control: A549. Primary Antibody (green line): Rabbit Anti-TAZ antibody (TMAB-01810) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 3 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Kidney (Rat) Lysate at 40 μg
Primary:
Anti-TAZ (TMAB-01810) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kDa
Observed band size: 52 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe transcriptional co-activator with PDZ-binding motif (TAZ) is a 14-3-3-binding molecule. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. TAZ may link events at the plasma membrane and cytosketeton to nuclear transcription in a manner that can be regulated by 14-3-3. TAZ shares homology with the WW domain of Yes-associated protein (YAP) and functions as a transcriptional co-activator by binding to the PPXY motif present on transcription factors. TAZ recognizes immunoreactive protein bands in lysates from MDCK, NIH-3T3 and 293T cells. In addition, COS7, Hep G2, CHO and HeLa cells express endogenous TAZ. 14-3-3 binding requires TAZ phosphorylation on a single Serine 89 residue, resulting in the inhibition of TAZ transcriptional co-activation through 14-3-3-mediated nuclear export.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TAZ
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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