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Anti-Synaptophysin/SYP Polyclonal Antibody

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Catalog No. TMAB-01802
Alias SYP, Synaptophysin, Major synaptic vesicle protein p38

Anti-Synaptophysin/SYP Polyclonal Antibody is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Synaptophysin/SYP Polyclonal Antibody

Anti-Synaptophysin/SYP Polyclonal Antibody

😃Good
Catalog No. TMAB-01802Alias SYP, Synaptophysin, Major synaptic vesicle protein p38
Anti-Synaptophysin/SYP Polyclonal Antibody is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Synaptophysin/SYP Polyclonal Antibody is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasSYP, Synaptophysin, Major synaptic vesicle protein p38
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (TMAB-01802) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (TMAB-01802) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
Cerebrum (mouse) Lysate at 40 μg
Primary: Anti-Synaptophysin (TMAB-01802) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
4. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (TMAB-01802) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Synaptophysin
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Synapse marker,SNAPs & SNAREs
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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