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Anti-Synaptophysin/SYP Polyclonal Antibody
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Catalog No. TMAB-01802
Alias SYP, Synaptophysin, Major synaptic vesicle protein p38
Anti-Synaptophysin/SYP Polyclonal Antibody is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Synaptophysin/SYP Polyclonal Antibody
Anti-Synaptophysin/SYP Polyclonal Antibody
😃Good
Catalog No. TMAB-01802Alias SYP, Synaptophysin, Major synaptic vesicle protein p38
Anti-Synaptophysin/SYP Polyclonal Antibody is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Synaptophysin/SYP Polyclonal Antibody is a Rabbit antibody targeting Synaptophysin/SYP. Anti-Synaptophysin/SYP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | SYP, Synaptophysin, Major synaptic vesicle protein p38 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (TMAB-01802) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (TMAB-01802) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Cerebrum (mouse) Lysate at 40 μg Primary: Anti-Synaptophysin (TMAB-01802) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Synaptophysin) Polyclonal Antibody, Unconjugated (TMAB-01802) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011] |
| Immunogen | KLH conjugated synthetic peptide: human Synaptophysin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Synapse marker,SNAPs & SNAREs |
| Molecular Weight | Theoretical: 34 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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