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Anti-SQSTM1/p62 Antibody (9J396)
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Catalog No. TMAB-01771
Anti-SQSTM1/p62 Antibody (9J396) is a Rabbit antibody targeting SQSTM1/p62. Anti-SQSTM1/p62 Antibody (9J396) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-SQSTM1/p62 Antibody (9J396)
Anti-SQSTM1/p62 Antibody (9J396)
😃Good
Catalog No. TMAB-01771
Anti-SQSTM1/p62 Antibody (9J396) is a Rabbit antibody targeting SQSTM1/p62. Anti-SQSTM1/p62 Antibody (9J396) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $154 | 7-10 days | |
| 50 μL | $271 | 7-10 days | |
| 100 μL | $487 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM Reactivity: Human,Rat,Mouse |
| Ig Type | IgG |
| Clone | 9J396 |
| Reactivity | Human,Rat,Mouse |
| Verified Activity | 1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (SQSTM1/p62) monoclonal Antibody, Unconjugated (TMAB-01771) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. 25 μg total protein per Lane of various lysates probed with p62 SQSTM1 monoclonal antibody, unconjugated (TMAB-01771) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 3. The HepG2 (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-p62 SQSTM1 antibody (TMAB-01771): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. 4. Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human stomach fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009] |
| Immunogen | A synthesized peptide: human Sequestosome 1 |
| Antigen Species | Human |
| Gene ID | 8878 |
| Uniprot ID | |
| Biology Area | Autophagosome,Autophagosome,Pol II Transcription,Autophagosome,Regulation,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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