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Anti-Siglec-3/CD33 Polyclonal Antibody
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Catalog No. TMAB-01688
Alias Siglec-3, gp67, CD33 molecule
Anti-Siglec-3/CD33 Polyclonal Antibody is a Rabbit antibody targeting Siglec-3/CD33. Anti-Siglec-3/CD33 Polyclonal Antibody can be used in ELISA,ICC/IF,IF,IHC-Fr,IHC-P,WB.
Anti-Siglec-3/CD33 Polyclonal Antibody
Anti-Siglec-3/CD33 Polyclonal Antibody
😃Good
Catalog No. TMAB-01688Alias Siglec-3, gp67, CD33 molecule
Anti-Siglec-3/CD33 Polyclonal Antibody is a Rabbit antibody targeting Siglec-3/CD33. Anti-Siglec-3/CD33 Polyclonal Antibody can be used in ELISA,ICC/IF,IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Siglec-3/CD33 Polyclonal Antibody is a Rabbit antibody targeting Siglec-3/CD33. Anti-Siglec-3/CD33 Polyclonal Antibody can be used in ELISA,ICC/IF,IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Siglec-3, gp67, CD33 molecule |
| Ig Type | IgG |
| Reactivity | Mouse |
| Verified Activity | Sample: Lane 1: Bone (Mouse) Lysate at 40 μg Lane 2: LympHnode (Mouse) Lysate at 40 μg Primary: Anti-CD33 (TMAB-01688) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/37 kDa Observed band size: 65 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Tissue Specificity | Monocytic/myeloid lineage cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome. [provided by Alliance of Genome Resources, Apr 2022] |
| Immunogen | KLH conjugated synthetic peptide: mouse CD33 |
| Antigen Species | Mouse |
| Gene Name | CD33 |
| Gene ID | |
| Protein Name | Myeloid cell surface antigen CD33 |
| Uniprot ID | |
| Biology Area | Myeloid Cells,Human Lineage Negative |
| Function | Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). |
| Molecular Weight | Theoretical: 40/37 kDa. Actual: 67 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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