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Anti-SAE2/UBA2 Polyclonal Antibody

Catalog No. TMAB-12473 Copy Product Info
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Anti-SAE2/UBA2 Polyclonal Antibody is a Rabbit antibody targeting SAE2/UBA2. Anti-SAE2/UBA2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-SAE2/UBA2 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-12473

Anti-SAE2/UBA2 Polyclonal Antibody is a Rabbit antibody targeting SAE2/UBA2. Anti-SAE2/UBA2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-SAE2/UBA2 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-SAE2/UBA2 Polyclonal Antibody is a Rabbit antibody targeting SAE2/UBA2. Anti-SAE2/UBA2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat (predicted:Human,Mouse,Dog,Horse,Rabbit)
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SAE2) Polyclonal Antibody, Unconjugated (TMAB-12473) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundPosttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SAE2
Antigen Species
Human
Gene Name
UBA2
Gene ID
Protein Name
SUMO-activating enzyme subunit 2
Uniprot ID
Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Chemical Properties
Molecular WeightTheoretical: 71 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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