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Anti-S6K1/RPS6KB1 Polyclonal Antibody
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Catalog No. TMAB-01647
Anti-S6K1/RPS6KB1 Polyclonal Antibody is a Rabbit antibody targeting S6K1/RPS6KB1. Anti-S6K1/RPS6KB1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-S6K1/RPS6KB1 Polyclonal Antibody
Anti-S6K1/RPS6KB1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01647
Anti-S6K1/RPS6KB1 Polyclonal Antibody is a Rabbit antibody targeting S6K1/RPS6KB1. Anti-S6K1/RPS6KB1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-S6K1/RPS6KB1 Polyclonal Antibody is a Rabbit antibody targeting S6K1/RPS6KB1. Anti-S6K1/RPS6KB1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Goat) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RPS6KB1) Polyclonal Antibody, Unconjugated (TMAB-01647) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: MCF-7 (Human) Cell Lysate at 30 μg U-2Os (Human) Cell Lysate at 30 μg MOLT-4 (Human) Cell Lysate at 30 μg Primary: Anti-RPS6KB1 (TMAB-01647) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 68 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013] |
| Immunogen | KLH conjugated synthetic peptide: human RPS6KB1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Rb,DNA Damage Recognition,Ribosome,Obesity,S6K Family |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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