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Anti-S100B Antibody (2F740)
😃Good
Catalog No. TMAB-01645
Alias S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF
Anti-S100B Antibody (2F740) is a Mouse antibody targeting S100B. Anti-S100B Antibody (2F740) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-S100B Antibody (2F740)
Anti-S100B Antibody (2F740)
😃Good
Catalog No. TMAB-01645Alias S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF
Anti-S100B Antibody (2F740) is a Mouse antibody targeting S100B. Anti-S100B Antibody (2F740) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-S100B Antibody (2F740) is a Mouse antibody targeting S100B. Anti-S100B Antibody (2F740) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF |
| Ig Type | IgG |
| Clone | 2F740 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 5. 25 μg total protein per Lane of various lysates probed with S100B monoclonal antibody, unconjugated (TMAB-01645) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 12. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 13. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 14. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 15. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 16. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 17. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with S100B Monoclonal Antibody, Unconjugated (TMAB-01645) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 18. The A375 (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Mouse Anti-S100B antibody (TMAB-01645): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Blank control (black): PBS. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. |
| Immunogen | Recombinant Protein: human S100B protein |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cell differentiation,Cell Differentiation,s100 beta,Astrocyte |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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