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Anti-RIPK1 Polyclonal Antibody

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Catalog No. TMAB-01620
Alias RIPK1, RIP1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1 (RIP-1), Cell death protein RIP

Anti-RIPK1 Polyclonal Antibody is a Rabbit antibody targeting RIPK1. Anti-RIPK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-RIPK1 Polyclonal Antibody

Anti-RIPK1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01620Alias RIPK1, RIP1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1 (RIP-1), Cell death protein RIP
Anti-RIPK1 Polyclonal Antibody is a Rabbit antibody targeting RIPK1. Anti-RIPK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-RIPK1 Polyclonal Antibody is a Rabbit antibody targeting RIPK1. Anti-RIPK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasRIPK1, RIP1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1 (RIP-1), Cell death protein RIP
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (RIPK-1) Polyclonal Antibody, Unconjugated (TMAB-01620) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (RIPK-1) Polyclonal Antibody, Unconjugated (TMAB-01620) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-RIPK-1 (TMAB-01620) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa
4. Sample:
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-RIPK-1 (TMAB-01620) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa
5. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RIPK1) Polyclonal Antibody, Unconjugated (TMAB-01620) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundEssential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human RIPK1
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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