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Anti-RIPK1 Polyclonal Antibody
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Catalog No. TMAB-01620
Alias RIPK1, RIP1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1 (RIP-1), Cell death protein RIP
Anti-RIPK1 Polyclonal Antibody is a Rabbit antibody targeting RIPK1. Anti-RIPK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-RIPK1 Polyclonal Antibody
Anti-RIPK1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01620Alias RIPK1, RIP1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1 (RIP-1), Cell death protein RIP
Anti-RIPK1 Polyclonal Antibody is a Rabbit antibody targeting RIPK1. Anti-RIPK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-RIPK1 Polyclonal Antibody is a Rabbit antibody targeting RIPK1. Anti-RIPK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | RIPK1, RIP1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1 (RIP-1), Cell death protein RIP |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (RIPK-1) Polyclonal Antibody, Unconjugated (TMAB-01620) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (RIPK-1) Polyclonal Antibody, Unconjugated (TMAB-01620) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-RIPK-1 (TMAB-01620) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 70 kDa 4. Sample: Raji (Human) Cell Lysate at 30 μg Primary: Anti-RIPK-1 (TMAB-01620) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 70 kDa 5. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RIPK1) Polyclonal Antibody, Unconjugated (TMAB-01620) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains. |
| Immunogen | KLH conjugated synthetic peptide: human RIPK1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 74 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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