Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-Rb Polyclonal Antibody

😃Good
Catalog No. TMAB-01611
Alias Retinoblastoma-associated protein, Retinoblastoma, RB1, RB transcriptional corepressor 1, RB, pRb (Rb), pRb, PPP1R130, pp110, p110-RB1, p105-Rb, OSRC

Anti-Rb Polyclonal Antibody is a Rabbit antibody targeting Rb. Anti-Rb Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Rb Polyclonal Antibody

Anti-Rb Polyclonal Antibody

😃Good
Catalog No. TMAB-01611Alias Retinoblastoma-associated protein, Retinoblastoma, RB1, RB transcriptional corepressor 1, RB, pRb (Rb), pRb, PPP1R130, pp110, p110-RB1, p105-Rb, OSRC
Anti-Rb Polyclonal Antibody is a Rabbit antibody targeting Rb. Anti-Rb Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5287-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-Rb Polyclonal Antibody is a Rabbit antibody targeting Rb. Anti-Rb Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasRetinoblastoma-associated protein, Retinoblastoma, RB1, RB transcriptional corepressor 1, RB, pRb (Rb), pRb, PPP1R130, pp110, p110-RB1, p105-Rb, OSRC
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Rb Polyclonal Antibody, Unconjugated (TMAB-01611) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Mouse Thymus tissue lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-Rb (TMAB-01611) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 135 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human P105 RB
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Rb family,Rb,Tumor Suppressors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-Rb Polyclonal Antibody | purchase Anti-Rb Polyclonal Antibody | Anti-Rb Polyclonal Antibody cost | order Anti-Rb Polyclonal Antibody