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Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody

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Catalog No. TMAB-01609
Alias tumor necrosis factor ligand superfamily member 11, TNFSF11, Receptor activator of nuclear factor κ-B ligand, Receptor activator of nuclear factor kappa-B ligand, RANK L, Osteoclast differentiation factor, OPGL, ODF, CD254

Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody is a Rabbit antibody targeting RANKL/TNFSF11/CD254. Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody

Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody

😃Good
Catalog No. TMAB-01609Alias tumor necrosis factor ligand superfamily member 11, TNFSF11, Receptor activator of nuclear factor κ-B ligand, Receptor activator of nuclear factor kappa-B ligand, RANK L, Osteoclast differentiation factor, OPGL, ODF, CD254
Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody is a Rabbit antibody targeting RANKL/TNFSF11/CD254. Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody is a Rabbit antibody targeting RANKL/TNFSF11/CD254. Anti-RANKL/TNFSF11/CD254 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Aliastumor necrosis factor ligand superfamily member 11, TNFSF11, Receptor activator of nuclear factor κ-B ligand, Receptor activator of nuclear factor kappa-B ligand, RANK L, Osteoclast differentiation factor, OPGL, ODF, CD254
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog)
Verified Activity
1. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (TMAB-01609) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-OPGL/RANKL/ODF Polyclonal Antibody, Unconjugated (TMAB-01609) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: mouse splenocytes (blue)
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μL in 100 μL 1X PBS containing 0.5% BSA (green).
4. Sample:
HUVEC-C Cell (Human) Lysate at 40 μg
HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-RANKL/CD254 (TMAB-01609) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 56 kDa
5. Sample:
LympHnode (Mouse) Lysate at 40 μg
Thymus (Mouse) Lysate at 40 μg
Primary: Anti-RANKL/CD254 (TMAB-01609) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37/50 kDa
Observed band size: 50 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm; Secreted and Cell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human OPGL
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Thrombosis,Regulatory T Cells,TNF Superfamily
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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