Anti-PTGER1 Polyclonal Antibody is a Rabbit antibody targeting PTGER1. Anti-PTGER1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat (predicted:Human,Dog)

1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PTGER1 Polyclonal Antibody, Unconjugated (TMAB-01591) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Sample: Brain (Rat) Lysate at 40 μg
Primary: Anti-Prostaglandin E Receptor EP1 (TMAB-01591) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
3. Sample: Kidney (Mouse) Lysate at 40 μg
Primary: Anti-Prostaglandin E Receptor EP1 (TMAB-01591) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Prostaglandin E Receptor EP1

Human

PTGER1

Prostaglandin E2 receptor EP1 subtype

GPCR,Prostaglandins