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Anti-Procalcitonin/CALCA Polyclonal Antibody 3 is a Rabbit antibody targeting Procalcitonin/CALCA. Anti-Procalcitonin/CALCA Polyclonal Antibody 3 can be used in IF,IHC-Fr,IHC-P.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-Procalcitonin/CALCA Polyclonal Antibody 3 is a Rabbit antibody targeting Procalcitonin/CALCA. Anti-Procalcitonin/CALCA Polyclonal Antibody 3 can be used in IF,IHC-Fr,IHC-P. |
Synonyms | Procalcitonin, PCT, KC, CT, CGRP-I, CGRP1, CGRP, calcitonin-related polypeptide α, calcitonin-related polypeptide alpha, CALC1 |
Ig Type | IgG |
Reactivity | Mouse,Rat (predicted:Human,Chicken,Pig,Cow) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CGRP-I) Polyclonal Antibody, Unconjugated (TMAB-01577) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CGRP-1 Polyclonal Antibody, Unconjugated (TMAB-01577) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CGRP-I) Polyclonal Antibody, Unconjugated (TMAB-01577) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration | 1 mg/mL |
Research Background | Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. |
Immunogen | KLH conjugated synthetic peptide: human CGRP-I |
Antigen Species | Human |
Gene Name | CALCA |
Gene ID | |
Protein Name | Calcitonin |
Uniprot ID | |
Biology Area | Hormones,Hormones,Hormones |
Function | The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 µg/L) of clinical assays. The level of procalcitonin rises in a response to a proinflammatory stimulus, especially of bacterial origin. It does not rise significantly with viral or non-infectious inflammations. With the derangements that a severe infection with an associated systemic response brings, the blood levels of procalcitonin may rise to 100 µg/L. In serum, procalcitonin has a half-life of 25 to 30 hours. Remarkably the high procalcitonin levels produced during infections are not followed by a parallel increase in calcitonin or a decrease in serum calcium levels. |
Molecular Weight | Theoretical: 3.5 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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