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Anti-PRMT5 Antibody (3P436)
😃Good
Catalog No. TMAC-03426
Alias SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5
Anti-PRMT5 Antibody (3P436) is a Rabbit antibody targeting PRMT5. Anti-PRMT5 Antibody (3P436) can be used in WB,ICC/IF,IHC.
Anti-PRMT5 Antibody (3P436)
Anti-PRMT5 Antibody (3P436)
😃Good
Catalog No. TMAC-03426Alias SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5
Anti-PRMT5 Antibody (3P436) is a Rabbit antibody targeting PRMT5. Anti-PRMT5 Antibody (3P436) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-PRMT5 Antibody (3P436) is a Rabbit antibody targeting PRMT5. Anti-PRMT5 Antibody (3P436) can be used in WB,ICC/IF,IHC. |
| Synonyms | SKB1Hs, SKB1, JBP1, IBP72, HSL7, HRMT1L5 |
| Ig Type | IgG |
| Clone | 3P436 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PRMT5 on A431 cell lysates using anti-PRMT5 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. 6. ICC staining PRMT5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The formation of the spliceosome includes the assembly of Sm proteins in an ordered manner onto snRNAs. This process is mediated by the survival of a motor neuron (SMN) protein and is enhanced by modification of specific Arginine residues in the Sm proteins to symmetrical dimethylarginines (sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome, a complex comprised of the type II methyltransferase PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation. The gene encoding human PRMT5 maps to chromosome 14q11. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 73 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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