Anti-PRKRA Antibody (9V632) is a Rabbit antibody targeting PRKRA. Anti-PRKRA Antibody (9V632) can be used in FCM, IF, IHC-Fr, IHC-P, WB.

IgG

9V632

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with PRKRA monoclonal antibody, unconjugated (TMAB-11747) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PRKRA antibody (TMAB-11747, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human PRKRA

Human

PRKRA

Interferon-inducible double-stranded RNA-dependent protein kinase activator A

Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.