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Anti-PRDM1 Antibody (4L235)
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Catalog No. TMAC-03412
Alias PRDM1_HUMAN, Prdm1, PRDM 1, PRDI-binding factor 1, PRDI-BF1, PRDI binding factor 1, PRDI BF1, PR domain-containing protein 1, PR domain zinc finger protein 1, PR domain containing protein 1, PR domain containing 1 with ZNF domain isoform 2, PR domain containing 1 with ZNF domain, PR Domain Containing 1, Positive regulatory domain I-binding factor 1, Positive Regulatory Domain I Binding Factor 1, BLIMP-1, BLIMP1, Beta-interferon gene positive regulatory domain I-binding factor, Beta interferon gene positive regulatory domain I binding factor, B Lymphocyte Induced Maturation Protein 1
Anti-PRDM1 Antibody (4L235) is a Mouse antibody targeting PRDM1. Anti-PRDM1 Antibody (4L235) can be used in FCM,ICC,WB.
Anti-PRDM1 Antibody (4L235)
Anti-PRDM1 Antibody (4L235)
😃Good
Catalog No. TMAC-03412Alias PRDM1_HUMAN, Prdm1, PRDM 1, PRDI-binding factor 1, PRDI-BF1, PRDI binding factor 1, PRDI BF1, PR domain-containing protein 1, PR domain zinc finger protein 1, PR domain containing protein 1, PR domain containing 1 with ZNF domain isoform 2, PR domain containing 1 with ZNF domain, PR Domain Containing 1, Positive regulatory domain I-binding factor 1, Positive Regulatory Domain I Binding Factor 1, BLIMP-1, BLIMP1, Beta-interferon gene positive regulatory domain I-binding factor, Beta interferon gene positive regulatory domain I binding factor, B Lymphocyte Induced Maturation Protein 1
Anti-PRDM1 Antibody (4L235) is a Mouse antibody targeting PRDM1. Anti-PRDM1 Antibody (4L235) can be used in FCM,ICC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $428 | 7-10 days |
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| Description | Anti-PRDM1 Antibody (4L235) is a Mouse antibody targeting PRDM1. Anti-PRDM1 Antibody (4L235) can be used in FCM,ICC,WB. |
| Synonyms | PRDM1_HUMAN, Prdm1, PRDM 1, PRDI-binding factor 1, PRDI-BF1, PRDI binding factor 1, PRDI BF1, PR domain-containing protein 1, PR domain zinc finger protein 1, PR domain containing protein 1, PR domain containing 1 with ZNF domain isoform 2, PR domain containing 1 with ZNF domain, PR Domain Containing 1, Positive regulatory domain I-binding factor 1, Positive Regulatory Domain I Binding Factor 1, BLIMP-1, BLIMP1, Beta-interferon gene positive regulatory domain I-binding factor, Beta interferon gene positive regulatory domain I binding factor, B Lymphocyte Induced Maturation Protein 1 |
| Clone | 4L235 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of PRDM1 on human PRDM1 recombinant protein using anti-PRDM1 antibody at 1/1,000 dilution. 2. Western blot analysis of PRDM1 on HEK293 (1) and PRDM1-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM1 antibody at 1/1,000 dilution. 3. ICC staining PRDM1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Raji cells with PRDM1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).     |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC: 1:200-1000; FCM: 1:100-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 92 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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