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Anti-PPM1G Antibody (3C482)
😃Good
Catalog No. TMAY-01796
Alias protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG
Anti-PPM1G Antibody (3C482) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (3C482) can be used in WB,IHC-P,ICC/IF.
Anti-PPM1G Antibody (3C482)
Anti-PPM1G Antibody (3C482)
😃Good
Catalog No. TMAY-01796Alias protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG
Anti-PPM1G Antibody (3C482) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (3C482) can be used in WB,IHC-P,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PPM1G Antibody (3C482) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (3C482) can be used in WB,IHC-P,ICC/IF. |
| Alias | protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3C482 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human PPM1G in human heart with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to cardiac muscle. 2. Immunochemical staining of human PPM1G in human epididymis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to skeletal muscle. 3. Immunofluorescence staining of Human PPM1G in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PPM1G monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). 4. Anti-PPM1G rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: 293T Whole Cell Lysate. -Lane C: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:59 kDa |
| Application | WB,IHC-P,ICC/IF |
| Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human PPM1G (rh PPM1G; TMPY-02197; O15355; Met317-Asp546). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein that belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human PPM1G protein (TMPY-02197) |
| Antigen Species | Human |
| Biology Area | Phosphatases and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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