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Anti-Porimin Polyclonal Antibody
😃Good
Catalog No. TMAB-01559
Alias Transmembrane protein 123, TMEM123, Serine/threonine rich receptor, Pro oncosis receptor inducing membrane injury, PORIM, Keratinocytes associated transmembrane protein 3, KCT3
Anti-Porimin Polyclonal Antibody is a Rabbit antibody targeting Porimin. Anti-Porimin Polyclonal Antibody can be used in WB,FCM.
Anti-Porimin Polyclonal Antibody
Anti-Porimin Polyclonal Antibody
😃Good
Catalog No. TMAB-01559Alias Transmembrane protein 123, TMEM123, Serine/threonine rich receptor, Pro oncosis receptor inducing membrane injury, PORIM, Keratinocytes associated transmembrane protein 3, KCT3
Anti-Porimin Polyclonal Antibody is a Rabbit antibody targeting Porimin. Anti-Porimin Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Porimin Polyclonal Antibody is a Rabbit antibody targeting Porimin. Anti-Porimin Polyclonal Antibody can be used in WB,FCM. |
| Alias | Transmembrane protein 123, TMEM123, Serine/threonine rich receptor, Pro oncosis receptor inducing membrane injury, PORIM, Keratinocytes associated transmembrane protein 3, KCT3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Sample: Skin (Mouse) Lysate at 40 μg Skin (Rat) Lysate at 40 μg Primary: Anti-Porimin (TMAB-01559) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kDa Observed band size: 64 kDa 2. Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-Porimin antibody (TMAB-01559) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Sample: Embryo (Mouse) Lysate at 40 μg Primary: Anti-Porimin (TMAB-01559) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kDa Observed band size: 74 kDa 4. Sample: LympHnode (Mouse) Lysate at 40 μg Primary: Anti-Porimin (TMAB-01559) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kDa Observed band size: 74 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 2ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti PORIMIN (pro oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death. |
| Immunogen | KLH conjugated synthetic peptide: human Porimin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 19 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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