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Anti-PMVK Polyclonal Antibody

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Catalog No. TMAB-11593

Anti-PMVK Polyclonal Antibody is a Rabbit antibody targeting PMVK. Anti-PMVK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PMVK Polyclonal Antibody

Anti-PMVK Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-11593
Anti-PMVK Polyclonal Antibody is a Rabbit antibody targeting PMVK. Anti-PMVK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-PMVK Polyclonal Antibody is a Rabbit antibody targeting PMVK. Anti-PMVK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PMVK Polyclonal Antibody, Unconjugated (TMAB-11593) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Uterus (Mouse) Lysate at 40 μg
Cerebrum (Mouse) Lysate at 40 μg
Heart (Mouse) Lysate at 40 μg
Lymph node (Mouse) Lysate at 40 μg
293T (Human) Cell Lysate at 30 μg
Primary: Anti-PMVK (TMAB-11593) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationPeroxisome.
Tissue SpecificityHeart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundPMVK is a 192 amino acid peroxisomal enzyme belonging to the nucleoside monophosphate (NMP) kinase family and is expressed in heart, liver, skeletal muscle, kidney, and pancreas with lower expression in brain, placenta and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway. PMVK exists as a monomer and is encoded by a gene located on human chromosome 1, which houses over 3,000 genes and is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PMVK
Antigen Species
Human
Gene Name
PMVK
Gene ID
Protein Name
phosphomevalonate kinase
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 22 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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