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Anti-PLCγ1 Antibody (8G514) (Synonyms: PLCy1, PLC-II, PLCgamma1, PLC148, PLC1, PLC γ1, PLC γ 1, PLC y1, ...)
Catalog No. TMAC-03370 Copy Product Info
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Anti-PLCγ1 Antibody (8G514) is a Mouse antibody targeting PLCγ1. Anti-PLCγ1 Antibody (8G514) can be used in FCM,ICC,IHC,WB.
Anti-PLCγ1 Antibody (8G514)
Copy Product Info🥰Excellent
Catalog No. TMAC-03370
Synonyms PLCy1, PLC-II, PLCgamma1, PLC148, PLC1, PLC γ1, PLC γ 1, PLC y1, PLC y 1, PLC gamma1, PLC gamma 1, NCKAP3, IDAA
Anti-PLCγ1 Antibody (8G514) is a Mouse antibody targeting PLCγ1. Anti-PLCγ1 Antibody (8G514) can be used in FCM,ICC,IHC,WB.
Anti-PLCγ1 Antibody
(8G514)
(8G514)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $297 | 7-10 days | 7-10 days | |
| 100 μL | $428 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
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| Description | Anti-PLCγ1 Antibody (8G514) is a Mouse antibody targeting PLCγ1. Anti-PLCγ1 Antibody (8G514) can be used in FCM,ICC,IHC,WB. |
| Synonyms | PLCy1, PLC-II, PLCgamma1, PLC148, PLC1, PLC γ1, PLC γ 1, PLC y1, PLC y 1, PLC gamma1, PLC gamma 1, NCKAP3, IDAA |
| Clone | 8G514 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PLCG1 on human PLCG1 recombinant protein using anti-PLCG1 antibody at 1/1,000 dilution. 2. Western blot analysis of PLCG1 on HEK293 (1) and PLCG1-hIgGFc transfected HEK293 (2) cell lysate using anti-PLCG1 antibody at 1/1,000 dilution. 3. Western blot analysis of PLCG1 on different cell lysate using anti-PLCG1 antibody at 1/1,000 dilution. Positive control: Line1: Hela, Line2: A431, Line3: C6, Line4: NIH/3T3, Line5: COS7, Line6: HCT116. 4. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin. 6. ICC staining PLCG1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).        |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:100-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Phospholipase C-gamma 1 (PLC g1) is an isozyme of the phosphoinositide-specific PLC family, which occupies a central role in hormonal signal transduction pathways and is a substrate for the epidermal growth factor receptor tyrosine kinase. Following activation of TrkB, PLC g1 is phosphorylated on Tyrosine 783, Tyrosine 771 and Tyrosine 1253. Tyrosine 783 lies just downstream of the kinase domain in a relatively short sequence motif characteristic of the Trk family of protein-tyrosine kinase receptors. The sequence around Tyrosine 783 fits a consensus sequence for binding PLC g1. PLC g1 also forms a complex with TrkB consistent with the possibility that one of the TrkB autophosphorylation sites provides a binding site for the PLC g1 SH2 domains, as is the case for other receptor protein-tyrosine kinases. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 148 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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