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Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731)

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Catalog No. TMAB-11514

Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731) is a Rabbit antibody targeting Placental alkaline phosphatase (PLAP). Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731)

Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731)

Copy Product Info
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Catalog No. TMAB-11514
Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731) is a Rabbit antibody targeting Placental alkaline phosphatase (PLAP). Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731) is a Rabbit antibody targeting Placental alkaline phosphatase (PLAP). Anti-Placental alkaline phosphatase (PLAP) Antibody (8R731) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Clone
8R731
Reactivity
Human
Verified Activity
1. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Placental alkaline phosphatase (PLAP) Monoclonal Antibody, Unconjugated (TMAB-11514) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Placental alkaline phosphatase (PLAP) Monoclonal Antibody, Unconjugated (TMAB-11514) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. 25 μg total protein per lane of various lysates (see on figure) probed with Placental alkaline phosphatase (PLAP) monoclonal antibody, unconjugated (TMAB-11514) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Lipid-anchor, GPI-anchor.
Tissue SpecificityTrace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundAlkaline phosphatase that can hydrolyze various phosphate compounds.
Antigen Details
Immunogen
A synthesized peptide: human ALPP
Antigen Species
Human
Gene Name
ALPP
Gene ID
Protein Name
Alkaline phosphatase, placental type
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 58 kDa. Actual: 70 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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