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Anti-PI3 kinase p150 Antibody (7M212)

Catalog No. TMAB-11429 Copy Product Info
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Anti-PI3 kinase p150 Antibody (7M212) is a Rabbit antibody targeting PI3 kinase p150. Anti-PI3 kinase p150 Antibody (7M212) can be used in WB,IHC-P,IHC-Fr,IF,FCM,ICC/IF.

Anti-PI3 kinase p150 Antibody (7M212)

Copy Product Info
🥰Excellent
Catalog No. TMAB-11429

Anti-PI3 kinase p150 Antibody (7M212) is a Rabbit antibody targeting PI3 kinase p150. Anti-PI3 kinase p150 Antibody (7M212) can be used in WB,IHC-P,IHC-Fr,IF,FCM,ICC/IF.

Anti-PI3 kinase p150 Antibody
(7M212)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1487-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4717-10 days7-10 days
In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PI3 kinase p150 Antibody (7M212) is a Rabbit antibody targeting PI3 kinase p150. Anti-PI3 kinase p150 Antibody (7M212) can be used in WB,IHC-P,IHC-Fr,IF,FCM,ICC/IF.
Ig Type
IgG
Clone
7M212
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with PI3 kinase p150 monoclonal antibody, unconjugated (TMAB-11429) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Concentration1mg/ml
Research BackgroundRegulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
Antigen Details
Immunogen
A synthesized peptide: human PI3 kinase p150
Antigen Species
Human
Gene Name
PIK3R4
Gene ID
Protein Name
Phosphoinositide 3-kinase regulatory subunit 4
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 153 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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