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Anti-PI 3 Kinase p85 beta Antibody (9A867) is a Rabbit antibody targeting PI 3 Kinase p85 beta. Anti-PI 3 Kinase p85 beta Antibody (9A867) can be used in WB,ICC/IF,IHC,IP,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-PI 3 Kinase p85 beta Antibody (9A867) is a Rabbit antibody targeting PI 3 Kinase p85 beta. Anti-PI 3 Kinase p85 beta Antibody (9A867) can be used in WB,ICC/IF,IHC,IP,FCM. |
Synonyms | PtdIns-3-kinase regulatory subunit p85-beta, PtdIns-3-kinase regulatory subunit beta, PtdIns 3 kinase p85 beta, PIK3R2, PIK3R 2, PI3-kinase subunit p85-beta, PI3-kinase regulatory subunit beta, PI3K regulatory subunit beta, PI3K, PI3 kinase p85 subunit beta, PI3 kinase p85 beta subunit, PI 3 Kinase p85 β, PI 3 Kinase p85 b, Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta), Phosphoinositide 3 kinase, regulatory subunit 2 (beta), Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta), Phosphoinositide 3 kinase regulatory subunit polypeptide 2, Phosphoinositide 3 kinase regulatory subunit 2 (beta), Phosphoinositide 3 kinase regulatory subunit 2, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta), Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2, Phosphatidylinositol 3 kinase regulatory subunit beta, Phosphatidylinositol 3 kinase regulatory beta subunit, Phosphatidylinositol 3 kinase, P85B_HUMAN, P85B, p85 beta, p85 |
Ig Type | IgG |
Reactivity | Human,Rat |
Verified Activity | 1. Western blot analysis of PI 3 Kinase p85 beta on different lysates using anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Raji, Lane 2: Hela, Lane 3: MCF-7, Lane 4: U937. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin. 5. ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with PI 3 Kinase p85 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110α expression is restricted to white blood cells. It has been shown to bind p85α and p85β, but it apparently does not phosphorylate these subunits. p110α seems to have the capacity to autophosphorylate. p110α does not interact with the p85 subunits. It has been shown to be activated by α and β heterotrimeric G proteins. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 82 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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