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Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody

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Catalog No. TMAB-01524

Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TORC2 (Ser171). Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody can be used in WB,FCM.

Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody

Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody

😃Good
Catalog No. TMAB-01524
Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TORC2 (Ser171). Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TORC2 (Ser171). Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig)
Verified Activity
1. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-TORC2 (Ser171) (TMAB-01524) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 95 kDa
2. Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (TMAB-01524)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (TMAB-01524) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010].
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Co-activators/co-repressors,Energy Metabolism,Cancer,Metabolism,Energy Metabolism,Co-activators/co-repressors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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