Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-PI3 kinase p85 alpha+gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated (TMAB-01479) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)/FITC Conjugated antibody (TMAB-01479-FITC)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature.

IHC-P,IHC-Fr,IF,FCM

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PI3 kinase p85 alpha around the phosphorylation site of Tyr467

Human

5295

Signal transduction,TLR Signaling,Lipid Kinases