Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-Phospho-p53 (Ser15) Antibody (5X80)
😃Good
Catalog No. TMAY-01535
Alias TRP53, p-p53 (Ser15), p-p53 (S15), Phospho-p53 (S15), p53 (p-Ser15), p53 (p-S15), P-53, LFS1, BMFS5, BCC7
Anti-Phospho-p53 (Ser15) Antibody (5X80) is a Rabbit antibody targeting Phospho-p53 (Ser15). Anti-Phospho-p53 (Ser15) Antibody (5X80) can be used in WB.
Anti-Phospho-p53 (Ser15) Antibody (5X80)
Anti-Phospho-p53 (Ser15) Antibody (5X80)
😃Good
Catalog No. TMAY-01535Alias TRP53, p-p53 (Ser15), p-p53 (S15), Phospho-p53 (S15), p53 (p-Ser15), p53 (p-S15), P-53, LFS1, BMFS5, BCC7
Anti-Phospho-p53 (Ser15) Antibody (5X80) is a Rabbit antibody targeting Phospho-p53 (Ser15). Anti-Phospho-p53 (Ser15) Antibody (5X80) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $80 | 7-10 days | |
| 100 μL | $235 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB Reactivity: Human |
| Alias | TRP53, p-p53 (Ser15), p-p53 (S15), Phospho-p53 (S15), p53 (p-Ser15), p53 (p-S15), P-53, LFS1, BMFS5, BCC7 |
| Ig Type | Rabbit IgG |
| Clone | 5X80 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from MCF-7, untreated (line A); treated with Doxorubicin (1 μM, 8 h), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p53 (Ser15) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) 2. Western blot analysis of extracts from MCF-7, untreated (-) or treated with Doxorubicin (1 μM, 8 h) (+), using Phospho-p53 (Ser15) Antibody, Rabbit Mab at 1:2000 dilution (upper) or Anti-p53 Antibody, Rabbit Monoclonal (middle) at 1:200 dilution or Beta-Tubulin Loading Control Antibody, Mouse Mab at 1:20000 dilution. |
| Application | WB |
| Recommended Dose | WB: 1:1000-1:5000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser15 of the Human Phospho-p53. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitin ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: residues around Ser15 of the Human Phospho-p53 |
| Antigen Species | Human |
| Biology Area | Apoptosis Transcription Factors and Regulators, Cancer Drug Targets, Tumor Suppressors |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-Phospho-p53 (Ser15) Antibody (5X80) | purchase Anti-Phospho-p53 (Ser15) Antibody (5X80) | Anti-Phospho-p53 (Ser15) Antibody (5X80) cost | order Anti-Phospho-p53 (Ser15) Antibody (5X80)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.