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Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody
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Catalog No. TMAB-01470
Alias p-NFKB1 (Ser337), p-NFKB1 (S337), Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p105 subunit, NFKB1 (p-Ser337), NFKB1 (p-S337), NFKB1, EBP-1, DNA-binding factor KBF1
Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFKB1 (Ser337). Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody
Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody
😃Good
Catalog No. TMAB-01470Alias p-NFKB1 (Ser337), p-NFKB1 (S337), Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p105 subunit, NFKB1 (p-Ser337), NFKB1 (p-S337), NFKB1, EBP-1, DNA-binding factor KBF1
Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFKB1 (Ser337). Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFKB1 (Ser337). Anti-Phospho-NFKB1 (Ser337) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | p-NFKB1 (Ser337), p-NFKB1 (S337), Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p105 subunit, NFKB1 (p-Ser337), NFKB1 (p-S337), NFKB1, EBP-1, DNA-binding factor KBF1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow) |
| Verified Activity | 1. Sample: Raji (Human) Cell Lysate at 30 μg Primary: Anti-Phospho-NFKB1 (Ser337) (TMAB-01470) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48/105 kDa Observed band size: 48 kDa 2. Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-NFKB1 (Ser337) antibody (TMAB-01470), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. 3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (TMAB-01470) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (TMAB-01470) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (TMAB-01470) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Sample: U87-MG (Human) Lysate at 30 μg MCF7 (Human) Lysate at 30 μg Primary: Anti-Phospho-NFKB1 (Ser337) (TMAB-01470) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48/105 kDa Observed band size: 48/105 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human NF KappaB p105 around the phosphorylation site of Ser337 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | p50,NFkB pathway,p50,NFkB pathway,Transcription Factors,Macrophage / Inflamm.,NFkB Pathway |
| Molecular Weight | Theoretical: 48/105 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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