Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse (predicted:Rat,Pig,Cow)

IgG

Human,Mouse (predicted:Rat,Pig,Cow)

1. Sample:
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-NFKB1 (Ser337) (TMAB-01470) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48/105 kDa
Observed band size: 48 kDa
2. Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-Phospho-NFKB1 (Ser337) antibody (TMAB-01470), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test.
3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (TMAB-01470) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (TMAB-01470) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-NFKB1 (Ser337)) Polyclonal Antibody, Unconjugated (TMAB-01470) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Sample:
U87-MG (Human) Lysate at 30 μg
MCF7 (Human) Lysate at 30 μg
Primary: Anti-Phospho-NFKB1 (Ser337) (TMAB-01470) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48/105 kDa
Observed band size: 48/105 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human NF KappaB p105 around the phosphorylation site of Ser337

Human

4790

p50,NFkB pathway,p50,NFkB pathway,Transcription Factors,Macrophage / Inflamm.,NFkB Pathway