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Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody
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Catalog No. TMAC-00109
Alias p-AKT1 (Ser129), p-AKT1 (S129), AKT1 (p-Ser129), AKT1 (p-S129)
Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Ser129). Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody can be used in WB.
Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody
Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody
😃Good
Catalog No. TMAC-00109Alias p-AKT1 (Ser129), p-AKT1 (S129), AKT1 (p-Ser129), AKT1 (p-S129)
Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Ser129). Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $218 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Ser129). Anti-Phospho-AKT1 (Ser129) Polyclonal Antibody can be used in WB. |
| Synonyms | p-AKT1 (Ser129), p-AKT1 (S129), AKT1 (p-Ser129), AKT1 (p-S129) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from A549 cells treated with PMA using Phospho-Akt (Ser129) antibody TMAC-00109. The lane on the right is treated with the antigen-specific peptide.  |
| Application | |
| Recommended Dose | WB: 1:500-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine129(D-N-S(p)-G-A) derived from Human Akt |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 65 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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